rnr [2019-07-10 11:42:01]

RNase R, required for protection against paraquate stress

Locus: BSU33610

Isoelectric point: 5.70

Molecular weight: 88.56 kDa

Protein length: 779 aa

Gene length: 2340 bp

Function: nonspecific degradation of rRNA

Product: exoribonuclease RNase R (EC 3.1.-.-)

Essential: no

Synonyms: yvaJ

Genomic Context

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Categories containing this gene/protein

• Metabolism → Nucleotide metabolism → Biosynthesis/ acquisition of nucleotides → Biosynthesis/ acquisition of nucleotides/ other
• Information processing → RNA synthesis and degradation → RNases → Exoribonucleases
• Lifestyles → Coping with stress → Resistance against oxidative and electrophile stress

Gene

Coordinates: 3,451,863 3,454,202

The protein

Catalyzed reaction/ biological activity

• 3'-5'-exoribonuclease

Protein family

• ribonuclease II (RNB) family (according to Swiss-Prot)

Domains

• S1 domain (aa 625 ... 708) (according to the Interpro database)

Structure

• 5XGU (from E. coli, 40% identity) PubMed

Expression and Regulation

Operons

• Genes: secG-yvaK-rnr-smpB-ssrA
  Description: PubMed

  Sigma factors

  • SigB: sigma factor, PubMed, in SigB regulon

  Regulation

  • yvaK: induced by stress (SigB) PubMed

• Genes: yvaK-rnr-smpB-ssrA
  Description: PubMed

  Regulation

  • yvaK: induced by stress (SigB) PubMed

Biological materials

Mutant

• MGNA-A495 (yvaJ::erm), available at the NBRP B. subtilis, Japan
• BKE33610 (rnr::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTCCATTATCCGACCTCCTG, downstream forward: _UP4_TAGCAGCTCAACCAGCAAAT
• BKK33610 (rnr::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTCCATTATCCGACCTCCTG, downstream forward: _UP4_TAGCAGCTCAACCAGCAAAT

Labs working on this gene/protein

• David Bechhofer, Mount Sinai School, New York, USA Homepage

References

Reviews

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Original publications

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